The purpose of this project was to develop an assay to explore the interactions between histone chaperone Anti-Silencing Function 1 (Asf1) and tetramers of histones (H3-H4)2. A successful assay was developed to work with flourescein-labeled proteins (Asf1 or histones) and explorations into interactions involving Asf1 protein were begun. Preliminary studies proved the assay was functional with Asf1 to Asf1 interplay assays and could be further used in investigations of histone chaperones and their importance within the life cycle and activity of cells.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Drohan, Lesley A

Contributors

• Ryder, Sean

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-011409-221736

Advisor

• Adams, David S.

Year

• 2009

Sponsor

• UMass Med

Date created

• 2009-01-14

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright