Genetic interaction studies have identified mechanisms controlling tissue specific gene expression, but with limited scalability. A high throughput image analysis platform was designed in conjunction with a genetic construct. The construct marks the intestinal cells in Caenorhabditis elegans, using mCherry, and indicates promoter of interest expression using GFP. The computational system creates a mask using the mCherry signal and quantifies GFP fluorescence, outputting quantitative changes in intensity within the intestine.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Shah, Akshaye Amish

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042519-123025

Advisor

• Ryder, Elizabeth F.

Year

• 2019

Sponsor

• University of Massachusetts Medical School

Date created

• 2019-04-25

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright