Understanding the Interactions Between FXR1 and PLC𝛃1

Fiorentino, Gabriella H.

Student Work

Understanding the Interactions Between FXR1 and PLC𝛃1 Public

The aim of this project is to understand the interactions between the proteins FXR1 and PLCβ1. It is known that the protein PLCβ1 will bind to FXR1, as FXR1 is a stress granule protein, but not much more of that relationship is known. Using neuronal PC12 cells, different techniques of gene overexpression and fluorescent imaging were used to observe both protein activity and localization. It was hypothesized that PLCβ1 may have specific interactions with FXR1 when subjected to a stressful environment. It was found through these experiments that FXR1 forms stress granules in response to specific stresses. Furthermore, PLCβ1 and FXR1 may not interact with one another directly, but possibly through other protein or RNA interactions.

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