Student Work
Examination of a putative Cu+ importer in Pseudomonas aeruginosa
PublicDownloadable Content
open in viewerThe focus of this project was to gain understanding on the putative copper importer PA5030 in the opportunistic bacteria Pseudomonas aeruginosa and its relationship to other proteins in the copper homeostasis network. First, recombination techniques were employed to successfully develop a double knockout mutant of the PA5030 gene and a different copper importer, PA1848 (ccoA). Second, in-silico exploration of the structure of the protein, accompanied by the development of a homolog filtering script, revealed two potential copper binding sites, M157-M173-H355 and C65-M185-M262, and 925 homologs that further suggest that PA5030 is a copper transporter.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042318-010932
- Award
- Advisor
- Year
- 2018
- Date created
- 2018-04-23
- Resource type
- Major
- Rights statement
- Last modified
- 2023-01-19
Relations
- In Collection:
Items
Items
Thumbnail | Title | Visibility | Embargo Release Date | Actions |
---|---|---|---|---|
Virginia_Nunez_Mir_MQP_Complete_Draft.pdf | Public | Download | ||
Filter_BLAST_Searches.zip | Public | Download |
Permanent link to this page: https://digital.wpi.edu/show/1r66j2561