The project's goal was to localize the HFR1 in the Arabidopsis thaliana seedling using immunohistochemical detection methods. HFR1 is a nuclear localized protein that affects light responses in the seedling. Tissue printing was a crude method used to roughly determine the conditions and seedling portion that would provide the greatest abundance of HFR1 protein. Microtome sections of the seedlings were then used with immunofluorescence to attempt to localize the HFR1 protein more precisely on a microscopic level.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Bowers, Rachel I.

Publisher

• Worcester Polytechnic Institute

Identifier

• 02D250M

Advisor

• Fairchild, Craig D.

Year

• 2002

Date created

• 2002-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright