The nu385 mutation affects nervous system development in Caenorhabditis elegans. The mutant phenotype resembles the dig-1 mutation, which is suspected to be involved in cell adhesion during axon migration in neuronal development. The C. elegans strain containing the nu385 mutation, KP980, was crossed with a Hawaiian isolate containing numerous single nucleotide polymorphisms (SNPs). Many of these SNPs produce restriction fragment length polymorphisms, which provided physical markers for mapping the mutation by genetic linkage analysis. This method allows for easy identification of recombination events within single animals by PCR expediting the mapping process. Our results suggest nu385 is linked to markers on the right arm of chromosome IV, approximately 13 map units from the center. Ultimately, these results will lead to cloning and characterization of nu385.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Morrison, Joshua Philip
• Black, Joshua Cranston

Publisher

• Worcester Polytechnic Institute

Identifier

• 02D340M

Advisor

• Ryder, Elizabeth F.

Year

• 2002

Date created

• 2002-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright