This project aims to develop a device and associated protocol to monitor supercooled red blood cell samples and detect when ice nucleation occurs. This device would minimize samples lost to current invasive testing methods for nucleation. A typical sample consists of a sedimented red blood cell layer, a supernatant layer, and a layer of mineral oil. When nucleation occurs, cells lyse and release hemoglobin; this causes the supernatant layer of the sample to change from a clear to a red color, which can be detected using a photometer. This photometer runs continuously with a vibration sensor and temperature sensor in order to monitor storage conditions. Through various testing methods, this device design was determined to be successful in detecting ice nucleation.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Woodland, Kathryn
• Gass, Madison
• Jolin, Grace
• Nearing, Isabel

Subject

• Project-Based Learning
• Sustainability
• Health
• Innovation
• Industry

Publisher

• Worcester Polytechnic Institute

Identifier

• 105626
• E-project-042623-211731

Keyword

• Monitoring System
• Continuous
• Red Blood Cells
• Remote
• Illuminance

Advisor

• Ambady, Sakthikumar
• Sabuncu, Ahmet Can

Year

• 2023

Sponsor

• Mass General Hospital
• Shriner's Hospital

UN Sustainable Development Goals

• 3 - Good Health and Well-being

Date created

• 2023-04-26

Resource type

• Major Qualifying Project

Major

• Mechanical Engineering
• Biomedical Engineering

Source

• E-project-042623-211731

Rights statement

• In Copyright

Last modified

• 2024-04-08