Etd
Synthetic Genes for Antimicrobial Peptides Public
The goal of this project was to clone and express the antimicrobial peptide protegrin 1 (PG-1). Initially a yeast system was chosen but was discarded due to technical difficulties. Invitrogen’s bacterial T7 expression system was chosen next to express the peptide. PG-1 expression was verified by anti-his immunoblot and then the peptide was purified by IMAC. Its activity was verified using a Bacillus subtillis radial diffusion assay.
- Last modified
- 11/27/2020
- Creator
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- Degree
- Unit
- Publisher
- Language
- English
- Identifier
- etd-0428103-102059
- Keyword
- Advisor
- Committee
- Defense date
- Year
- 2003
- Date created
- 2003-04-28
- Resource type
- Rights statement
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new_masters.pdf | 2019-06-28 | Public |
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