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Synthetic Genes for Antimicrobial Peptides Public

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The goal of this project was to clone and express the antimicrobial peptide protegrin 1 (PG-1). Initially a yeast system was chosen but was discarded due to technical difficulties. Invitrogen’s bacterial T7 expression system was chosen next to express the peptide. PG-1 expression was verified by anti-his immunoblot and then the peptide was purified by IMAC. Its activity was verified using a Bacillus subtillis radial diffusion assay.

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  • 11/27/2020
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  • English
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  • etd-0428103-102059
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  • 2003
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  • 2003-04-28
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