Alzheimer’s disease (AD) is a neurodegenerative disorder that leads to dementia in adults. The mechanism of neurodegeneration is thought to involve the extracellular production of a highly toxic A-beta peptide that engages cell surface receptors to induce cellular oxidative stress and apoptosis, but the signal transduction pathways that lead to A-beta induced cell death are unknown. We previously showed that a human ependymin neurotrophic peptide mimetic (hEPN-1) can promote cell survival in an in vitro AD model system. This initial observation was extended in this thesis by investigating the mechanism of A-beta induced apoptosis and hEPN-1 induced survival. Immunoblots were used to assay the total cellular levels of specific caspase proteins. The results show that A-beta induced apoptosis uses an extrinsic caspase pathway involving caspases-2 and -3, and that hEPN-1 treatment can reduce those caspase levels. A caspase activity assay showed that A-beta increased caspase-3/7 activity, while hEPN-1 treatment lowered it. Moreover, in vivo studies with AD transgenic mice showed that hEPN-1 treatment increased antioxidative superoxide dismutase levels in brain. Thus, hEPN-1 holds potential as a therapeutic to treat the underlying neurodegenerative cause of AD, not merely its symptoms as with other currently approved AD drugs.

Creator

• kapoor, varun

Colaboradores

• Gibson Daniel III
• Adams, David S.
• Ryder, Elizabeth F.

Degree

• MS

Unit

• Biology & Biotechnology

Publisher

• Worcester Polytechnic Institute

Language

• English

Identifier

• etd-071607-181533

Palabra Clave

• Caspases
• SOD
• Ependymins
• Alzheimer's

Advisor

• Adams, David S.

Committee

• Ryder, Elizabeth
• Gibson Daniel G., III

Defense date

• 2007-07-18

Year

• 2007

Date created

• 2007-07-16

Resource type

• Thesis

Rights statement

• In Copyright