Analysis of the Influence of Cellular Phase on Cell Traction Force Magnitudes Public
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Cell traction force is generated in the cytoskeleton by actomyosin activity and plays an important role in many cellular processes. In previous cell traction force experiments performed by our lab, unexpectedly large variations were measured. Because these experiments were utilizing a cell population of randomized phase, and there had been no documented investigation into whether cell phase affected cell traction force generation or propagation, it was hypothesized that there would be a significant difference in traction force between S phase and the other phases of interphase, as the physical and chemical changes happening within the nucleus at this time might elicit changes within the cytoskeleton. To test this hypothesis, we characterized the time-evolution of traction forces from a population of synchronized 3T3 fibroblasts. 3T3 fibroblasts were synchronized in G1-phase via serum starvation. The transition times between cellular phases during the first cell cycle after synchronization were identified by BrdU and Hoechst staining at different time points. After phase transition times were approximated, the traction forces of 9 cells were measured in 4-hour intervals for 24 hours. The differences between traction forces measured in G1, S, and G2 phases are not significant, demonstrating that cellular phase does not significantly affect traction force magnitude.
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